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目的:从金樱根中分离提取金樱根多糖,并探索其抗肿瘤活性。方法:水提醇沉法提取,Sevag法除蛋白,大孔树脂脱色后获得金樱根多糖粗品。建立小鼠S180肉瘤腹水瘤模型,并将小鼠随机分为4组:生理盐水组(阴性对照组)、金樱根多糖低、中、高剂量组(50,100,200 mg·kg-1)。建立移植实体瘤模型,将小鼠随机分为8组:生理盐水组(阴性对照组)、阳性对照5-Fu(5-氟脲嘧啶)组(20 mg·kg-1)、金樱根多糖低、中、高剂量组(50,100,200 mg·kg-1)、金樱根多糖各剂量组+5-Fu组。腹腔注射给药结束后处死小鼠,计算各用药组抑瘤率、生命延长率和白细胞数、胸腺指数、脾指数,并用EILSA法测定荷瘤小鼠血清中IL-2(白细胞介素-2)、TNF-α(肿瘤坏死因子-α)水平。结果:制备得到金樱根多糖粗品含糖量为85.43%。金樱根多糖50,100,200 mg·kg-1剂量组荷瘤小鼠生命延长率分别为3.07%±2.35%,10.11%±4.32%,34.3%±8.31%,与对照组相比,仅高剂量组有统计学意义(P<0.05)。但金樱根多糖低、中、高剂量组与5-Fu组(20 mg·kg-1)合用抑瘤率分别为62.74%±4.35%,67.92%±3.24%,78.79%±6.23%,明显优于单用5-Fu(P<0.05),且与单用5-Fu组相比合用能升高白细胞数、胸腺指数、脾指数及IL-2水平(P<0.05),但对TNF-α水平无影响。结论:金樱根多糖单用不能有效抑制小鼠S180肉瘤生长,但与5-Fu合用有明显的增效减毒作用。
OBJECTIVE: To isolate and purify the gold sap root polysaccharide from Jin Ying root and explore its anti-tumor activity. Methods: Water extraction and alcohol precipitation extraction, Sevag deproteinization, macroporous resin decolorization to obtain crude saurin polysaccharide. The mouse S180 sarcoma model was established. The mice were randomly divided into 4 groups: normal saline group (negative control group), low, medium and high dose of Sakura root polysaccharide (50,100,200 mg · kg-1). The model of transplantation solid tumor was established. The mice were randomly divided into 8 groups: normal saline group (negative control group), positive control 5-Fu (5-fluorouracil) group (20 mg · kg- Middle-dose and high-dose groups (50,100,200 mg · kg-1), each dose group of sakura root polysaccharides + 5-Fu group. The mice were sacrificed at the end of intraperitoneal injection, and the inhibition rate of tumor, life prolongation, white blood cell count, thymus index and spleen index were calculated. The levels of IL-2 (interleukin-2 ), TNF-α (tumor necrosis factor-α) levels. Results: The crude polysaccharides content was 85.43%. Compared with the control group, only the high-dose group had statistic significance (P <0.05), and the prolongation rate of the mice in tumor-bearing mice treated with 50,100,200 mg · kg-1 was 3.07% ± 2.35%, 10.11% ± 4.32% and 34.3% ± 8.31% Significance (P <0.05). However, the antitumor rates of Jinying root polysaccharide combined with 5-Fu group (20 mg · kg-1) were 62.74% ± 4.35%, 67.92% ± 3.24%, 78.79% ± 6.23% Compared with 5-Fu alone, the number of white blood cells, thymus index, spleen index and IL-2 level increased (P <0.05), but the level of TNF-α no effect. CONCLUSION: Golden sain root polysaccharide alone can not effectively inhibit the growth of S180 sarcoma in mice, but it has a synergistic and attenuating effect compared with 5-Fu.