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目的应用代谢组学研究技术测试与比较正常对照组和大鼠溃疡性结肠炎模型(Ulcerative ColitisModel,UCM)组结肠组织代谢产物,探讨UCM结肠组织中UC相关的特异性分子标志物。方法将Wistar大鼠随机分为正常对照组(22只)和溃疡性结肠炎模型组(21只),分笼喂养,采用2,4-二硝基氯苯(2,4-dinitrochloro-benzene,DNCB)复合乙酸法复制大鼠溃疡性结肠炎动物模型,收集两组大鼠结肠组织,进行1H-NMR图谱测定和主成分分析。结果与正常对照组大鼠相比,UC模型组结肠组织的内源性代谢谱、肠组织的代谢表型发生了显著变化,UC模型组大鼠结肠组织中丙酸、乳酸、牛磺酸、乙酸、脯氨酸、丙氨酸等小分子代谢物含量明显增加,差异有统计学意义(P<0.05)。结论 UC大鼠结肠组织的代谢谱明显偏离正常大鼠结肠组织,乳酸、牛磺酸、脯氨酸、丙氨酸等6种代谢物在临床上可作为溃疡性结肠炎早期诊疗的分子标志物。
OBJECTIVE: To test and compare the metabolites of colonic tissue in normal control group and Ulcerative Colitis Model (UCM) with the technique of metabonomics to explore the specific molecular markers associated with UC in UCM colon. Methods Wistar rats were randomly divided into normal control group (n = 22) and ulcerative colitis model group (n = 21). The rats were divided into groups and fed with 2,4-dinitrochlorobenzene DNCB) complex acetic acid method of rat ulcerative colitis animal model, colonic tissue of two groups were collected, 1H-NMR spectra and principal component analysis. Results Compared with the normal control group, the endogenous metabolic profile of colonic tissue and the metabolic phenotype of intestinal tissue in UC model group were significantly changed. In UC model group, propionic acid, lactic acid, taurine, Acetic acid, proline, alanine and other small molecule metabolites increased significantly, the difference was statistically significant (P <0.05). Conclusions The metabolic profile of colonic tissue of UC rats is obviously deviated from that of normal rats. Six metabolites such as lactate, taurine, proline, and alanine can be clinically used as molecular markers for the early diagnosis and treatment of ulcerative colitis .