论文部分内容阅读
采用96孔板微量稀释法,对从水杨梅中分离纯化得到的化合物的抑菌活性进行研究,测定获得了化合物对大肠杆菌、绿脓杆菌、乳链球菌、金黄色葡萄球菌、枯草芽孢杆菌的最小抑菌浓度(MIC).结果表明,水杨梅中得到的β-谷甾醇、熊果酸、山奈酚、槲皮素、山奈酚-3-O-β-D-葡萄糖苷、槲皮素-3-O-β-D-葡萄糖苷和胡萝卜苷对测试的5种细菌具有不同的抑制作用,其中山奈酚对大肠杆菌的最小抑菌浓度达到7.8μg/mL.并对得到的化合物山奈酚、槲皮素、山奈酚-3-O-β-D-葡萄糖苷、槲皮素-3-O-β-D-葡萄糖苷以及从其它植物样品中提取分离到的芹菜素、木犀草素、芹菜素-7-O-β-D-葡萄糖苷、木犀草素-7-O-β-D-葡萄糖苷的抗氧化活性进行研究,以抗坏血酸和BHT为对照,采用DPPH法测定自由基清除率,结果表明,水杨梅中分离得到的黄酮醇类化合物具有良好的抗氧化性,黄酮类化合物的抗氧化能力由强到弱顺序为槲皮素>槲皮素-3-O-β-D-葡萄糖苷>山奈酚>木犀草素>木犀草素-7-O-β-D-葡萄糖苷>山奈酚-3-O-β-D-葡萄糖苷>芹菜素>芹菜素-7-O-β-D-葡萄糖苷.
The antibacterial activities of the compounds isolated and purified from Myrica rubra were studied by 96-well plate microdilution method. The antibacterial activities of the compounds against Escherichia coli, Pseudomonas aeruginosa, Streptococcus lactis, Staphylococcus aureus and Bacillus subtilis The results showed that β-sitosterol, ursolic acid, kaempferol, quercetin, kaempferol-3-O-β-D-glucoside, quercetin- 3-O-β-D-glucoside and daucosterol had different inhibitory effects on the five tested bacteria, in which the minimum inhibitory concentration of kaempferol to Escherichia coli was 7.8 μg / mL, and the obtained compounds kaempferol, Quercetin, kaempferol-3-O-β-D-glucoside, quercetin-3-O-β-D-glucoside as well as the extraction and isolation of apigenin, luteolin and celery from other plant samples 7-O-β-D-glucoside and luteolin-7-O-β-D-glucoside were studied. Ascorbic acid and BHT were used as control. The free radical scavenging activity was determined by DPPH method. The results showed that flavonols isolated from Myrica rubra had good anti-oxidative activity, anti-oxidant activity of flavonoids The order of their ability to degrade is quercetin> quercetin -3-O-β-D-glucoside> kaempferol> luteolin> luteolin-7-O-β-D-glucoside> Phenol-3-O-β-D-glucoside> apigenin> apigenin-7-O-β-D-glucoside.