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目的比较分析乙肝肝硬化患者与正常人远端肠道微生物群的差异,探讨乙肝肝硬化与肠道微生物群变化的关系。方法选取11例乙肝肝硬化患者及20例正常人,提取其肠道微生物元基因组,采用高通量Solexa测序方法,通过基因比对、主成分分析、属种定量及功能代谢组成等生物信息学分析方法,找出疾病相关的属种和基因信息。结果与结论属种定量及组成分析发现,乙肝肝硬化患者存在不同程度的肠道菌群失调,表现为肠杆菌科(尤其是大肠杆菌、肺炎克雷伯菌和志贺菌属)以及韦荣球菌属富集(P<0.05),拟杆菌属缺失(P<0.05);功能及代谢分析发现,乙肝肝硬化患者富集较多的执行氨基酸和碳水化合物膜转运及代谢功能的基因。
Objective To compare the difference of distal gut microflora between patients with hepatitis B cirrhosis and normal subjects and to explore the relationship between hepatitis B cirrhosis and intestinal microflora. Methods Eleven patients with hepatitis B cirrhosis and 20 healthy controls were enrolled in this study. The gut microbiome was extracted and sequenced. By using high-throughput Solexa sequencing, the bioinformatics methods including gene alignment, principal component analysis, Analytical methods to identify disease-related genus and gene information. RESULTS AND CONCLUSIONS The quantitative and compositional analysis of the genus showed that there were different degrees of intestinal flora imbalance in patients with hepatitis B cirrhosis, which was characterized by Enterobacteriaceae (especially Escherichia coli, Klebsiella pneumoniae and Shigella) and Veillonella (P <0.05), and the genus Bacteroides was absent (P <0.05). The functional and metabolic analysis revealed that patients with hepatitis B cirrhosis were more likely to be enriched in genes that perform amino acid and carbohydrate membrane transport and metabolism.