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目的调查青海省海西州德令哈地区蚊虫及蚊媒病毒的种类及分布。方法在德令哈地区采集蚊虫标本,蚊虫标本经实验室常规处理后使用C6/36、BHK-21和Vero细胞开展病毒分离及9种病毒特异性基因检测及分析。结果2013年在德令哈地区共采集蚊虫6050只,分别为黄背伊蚊和里海伊蚊,其构成比分别为92.6%(5600/6050)和7.4%(450/6050)。所有蚊虫标本分为96批,接种上述3种细胞,这些蚊虫标本在BHK-21和Vero细胞上均未出现病变也未检测病毒基因阳性。但有32批蚊虫标本接种的C6/36细胞上清液和对应蚊虫标本研磨液中均检测到辽宁病毒(liaoningvirus,LNV),其中黄背伊蚊27批,里海伊蚊5批。病毒基因进化分析发现德令哈市分离的LNV与我国在新疆分离的LNV处在同一进化分支。结论 2013年青海省德令哈地区黄背伊蚊为优势蚊种,黄背伊蚊和里海伊蚊标本中LNV感染率均较高,为首次在当地发现LNV。
Objective To investigate the species and distribution of mosquitoes and mosquito vectors in Delingha, Haixi Prefecture, Qinghai Province. Methods Mosquitoes samples were collected in Delingha area. The mosquitoes were routinely isolated in the laboratory and were isolated by using C6 / 36, BHK-21 and Vero cells. Nine virus-specific genes were detected and analyzed. Results In 2013, a total of 6,050 mosquitoes were collected in the Delingha region, which were Aedes albopictus and Aedes albopictus respectively, with the proportions of 92.6% (5600/6050) and 7.4% (450/6050), respectively. All the mosquito specimens were divided into 96 batches and inoculated with the above three kinds of cells. The specimens of these mosquitoes did not appear to be pathological nor positive for the virus gene in BHK-21 and Vero cells. However, liaoningvirus (LNV) was detected in the supernatant of C6 / 36 cells inoculated with 32 mosquito specimens and in the polishing solution of the corresponding mosquito specimens, including 27 batches of Aedes aegypti and 5 batches of Aedes albopictus. The evolutionary analysis of viral genes found that LNV isolated in Delingha City is at the same evolutionary branch as LNV separated in Xinjiang. Conclusions In 2013, the Aedes albopictus in Delingha region of Qinghai Province was the dominant mosquito species. LNV infection rates were high in the specimens of Aedes albopictus and Aedes albopictus, which was the first time that LNV was found in the area.