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目的 观察雷公藤多甙 (MT)体外对成骨细胞 (OB)功能表达的影响 ,以进一步探讨雷公藤 (TW)致女性骨质疏松的机制。方法 分离新生SD大鼠头盖骨OB进行原代培养 ,经 5～ 6d至汇合 ,传代后加入不同浓度MT ,用对硝基苯磷酸法和考马斯亮蓝法作碱性磷酸酶 (ALP)比活性测定 ,茜素红染色法作钙化结节计数测定OB矿化能力。结果 培养 6d ,MT 1μg/ml起显著抑制OBALP比活性 (P <0 0 5 ) ;培养 2周 ,MT 10 -4 μg/ml起显著抑制OB矿化能力 (P <0 0 5 ) ;药物对OB功能表达的抑制程度与其剂量密切相关 (P <0 0 5 ,P <0 0 1)。结论 MT显著抑制OB功能表达且呈剂量依赖性。TW对OB的直接抑制作用 ,是其导致药物性骨质疏松的一个重要原因。
Objective To observe the effects of tripterygium glycosides (MT) on osteoblasts (OB) in vitro and to explore the mechanism of female osteoporosis caused by Tripterygium wilfordii (TW). METHODS: The skulls of newborn SD rats were isolated and cultured for primary culture. After 5 ~ 6 days, the MTs were added into the skulls of different concentrations. MT was assayed by the method of p-nitrophenyl phosphate and the specific activity of alkaline phosphatase (ALP) Alizarin red staining was used to determine the mineralization ability of OB. Results After cultured for 6 days, MT 1μg / ml significantly inhibited the specific activity of OBALP (P <0.05). After cultured for 2 weeks, MT 10-4 μg / ml significantly inhibited the mineralization of OB (P <0.05) The inhibition of OB function expression was closely related to its dose (P <0.05, P <0.01). Conclusion MT significantly inhibited the expression of OB in a dose-dependent manner. The direct effect of TW on the inhibition of OB is an important reason for the drug-induced osteoporosis.