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目的:研究p150Sal2在人卵巢癌细胞株中对MX1基因表达的影响。方法:将MX1基因的启动子克隆至带有Luciferase报告基因的pGL3-basic载体中,将其与p150Sal2表达载体用PEI介导的转染方法共转染至卵巢癌细胞株SKOV3,用双荧光素酶检测系统检测MX1启动子活性变化;将p150Sal2表达载体转染至卵巢癌细胞株ES-2,用western blot检测细胞中MX1基因的表达变化。结果:双荧光素酶检测系统检测MX1启动子活性被p150Sal2上调,western blot检测转染p150Sal2后细胞中MX1表达量增加。结论:p150Sal2在人卵巢癌细胞株中促进MX1基因的表达。
AIM: To investigate the effect of p150Sal2 on MX1 gene expression in human ovarian cancer cell lines. METHODS: The promoter of MX1 gene was cloned into the pGL3-basic vector carrying Luciferase reporter gene and co-transfected with p150Sal2 expression vector into ovarian cancer cell line SKOV3 using PEI-mediated transfection method. The activity of MX1 promoter was detected by enzyme detection system. The p150Sal2 expression vector was transfected into ovarian cancer cell line ES-2, and the expression of MX1 gene was detected by western blot. Results: The activity of MX1 promoter was detected by dual luciferase assay system. The expression of MX1 was increased in p150Sal2 cells transfected with p150Sal2 by western blot. Conclusion: p150Sal2 promotes MX1 gene expression in human ovarian cancer cell lines.