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结合连续加料降低聚合体系的放热量,及强化体系的换热能力等措施,实现了无搅拌模式聚合体系中温度分布的均一化。通过单次聚合过程构造了体积为38mL的大体积整体固定相。SEM及孔隙率分布测试证明了整体固定相具有均匀的孔结构,动态容量测定及分离蛋白质的实验,表明所制备的径向流动模式整体柱的容量及分辨率不依赖于流速,同时在高流速下具有低背压的特征,可用于生物大分子的分离制备。
Combined with continuous feed to reduce the heat release of the polymerization system, and to strengthen the heat transfer capacity of the system and other measures to achieve a uniform polymerization system without stirring the temperature distribution. A large bulk monolith with a volume of 38 mL was constructed by a single polymerization process. The SEM and porosity distribution tests demonstrated that the monolithic stationary phase has uniform pore structure, dynamic capacity and protein separation experiments. The results show that the capacity and resolution of the monolithic columns prepared by radial flow are not dependent on the flow rate, Under low back pressure features, can be used for the separation of biological macromolecules.