论文部分内容阅读
由于没有明确的形态差异 ,香菇的 3种不亲和交配反应即A =B =,A =B≠和A≠B =难以互相区别。本研究将OWE SOJ技术及核迁移试验用于香菇的交配型分析。结果表明 ,应用OWE SOJ技术 ,可以观察到 3种不同的菌落形态 :由A≠B≠配对形成的具锁状联合的绒毛状菌落 ,由A≠B =配对形成的无锁状联合的栅栏型菌落及由A =B =或A =B≠配对形成的无锁状联合的绒毛状菌落。通过一个附加的常规交配试验 ,A =B =和A=B≠反应易于互相区别开来。核迁移试验表明 ,核迁移出现于A =B≠反应中而不出现于A≠B =反应中。这一结果与OWE SOJ试验的结果是一致的。因此 ,在常规交配型分析的基础上 ,应用OWE SOJ技术及核迁移试验以准确地鉴定样本中所有单核体的交配型因子是可行的。
Because there is no clear morphological differences, three incompatible mash reactions Mushroom A = B =, A = B ≠ and A ≠ B = difficult to distinguish each other. In this study, OWE SOJ technology and nuclear migration test were applied to the mating type analysis of shiitake mushrooms. The results showed that three different colony morphologies were observed using the OWE SOJ technique: a villous colony with a combination of locks formed by A ≠ B ≠ pairing, a lock-free combined fence type formed by A ≠ B = pairing Colonies and lock-free, united villous colonies formed by the pairing of A = B = or A = B ≠. Through an additional routine mating experiment, A = B = and A = B ≠ reactions are easily distinguished from one another. Nuclear migration experiments show that nuclear migration occurs in the A = B ≠ reaction and does not occur in the A ≠ B = reaction. This result is consistent with the OWE SOJ test. Therefore, based on the conventional mating analysis, it is feasible to apply the OWE SOJ technique and the nuclear migration assay to accurately identify mating type factors for all monokaryons in the sample.