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为观察败血症时心肌肌浆网（ＳＲ）和核被膜（ＮＥ）的ｒｙａｎｏｄｉｎｅ受体的变化，采用结扎及穿刺盲肠（ＣＬＰ）制作败血症动物模型，用密度梯度离心分离ＳＲ和ＮＥ，用放射配体结合法研究ｒｙａｎｏｄｉｎｅ受体的特征。结果表明，大鼠早期败血症（ＣＬＰ后９ｈ）时，ＳＲ的ｒｙａｎｏｄｉｎｅ受体的最大结合（Ｂｍａｘ）增加２３％，ＮＥ的ｒｙａｎｏｄｉｎｅ受体的Ｂｍａｘ则增加１倍，二者比值降低３９％（Ｐ＜００１）；在晚期败血症（ＣＬＰ后１８ｈ）时，ＳＲｒｙａｎｏｄｉｎｅ受体的Ｂｍａｘ降低了３８％，ＮＥ的ｒｙａｎｏｄｉｎｅ受体的Ｂｍａｘ增加１６倍，二者比值降低７６％；ＳＲ和ＮＥｒｙａｎｏｄｉｎｅ受体的离解常数无显著改变。败血症时，ＳＲｒｙａｎｏｄｉｎｅ受体早期上调，晚期下调，而ＮＥｒｙａｎｏｄｉｎｅ受体均上调，这些变化可能与休克时相有关。
To observe the changes of ryanodine receptors in sarcoplasmic reticulum (SR) and nuclear envelope (NE) of sepsis in rabbits, animal models of sepsis were made by ligation and puncture cecum (CLP). SR and NE were separated by density gradient centrifugation. Study on the characteristics of ryanodine receptor by binding assay. The results showed that in rats with early sepsis (9h after CLP), the maximal binding of ryanodine receptor (Bmax) was increased by 23% in SR and the Bmax in ryanodine receptor in NE increased by 1 time (P < 001). In advanced sepsis (18h after CLP), the Bmax of SRryanodine receptor decreased by 38% and the NE of ryanodine receptor increased by 16 times and the ratio of them decreased by 76%. The SR and NEryanodine receptor The dissociation constants did not change significantly. In sepsis, SRryanodine receptors were up-regulated early, lately reduced, and NEryanodine receptors were up-regulated. These changes may be related to the phase of shock.