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建立双抗体夹心酶联免疫吸附法(ELISA)检测血清中骨膜素水平及初步应用于临床消化系肿瘤的检测。采用ELISA技术,利用双抗体夹心法制备骨膜素检测试剂盒,并进行自制试剂盒的指标评价。结果:双抗夹心ELISA法检测骨膜素的最小检出限为4.803 ng/ml,标准曲线在(4.803~500)ng/ml范围内线性良好。平均批内变异系数为7.2%,平均批间变异系数为14.1%,回收率为84.5%。278例胃肠道腺癌患者的血清骨膜素浓度为(40.9±15.4)ng/ml,显著高于63名健康对照者[(21.0±7.3)ng/ml,P<0.0001]和56例胃肠道腺瘤或息肉患者[(22.4±8.5)ng/ml,P<0.0001]。骨膜素ELISA试剂盒具有良好的灵敏性、特异性和准确性,今后有望用于临床消化系肿瘤的检测。
To establish a dual antibody sandwich enzyme-linked immunosorbent assay (ELISA) for detection of serum periostin levels and preliminary application in the detection of clinical gastrointestinal tumors. Using the technique of ELISA, the periostin assay kit was prepared by the double antibody sandwich method and the index of the kit was evaluated. Results: The minimum detectable limit of detection of periostin by double-antibody sandwich ELISA was 4.803 ng / ml and the calibration curve was linear over the range of (4.803-500) ng / ml. The average intra-assay CV was 7.2%, the average inter-assay CV was 14.1% and the recovery was 84.5%. The serum periostin concentrations in 278 patients with gastrointestinal adenocarcinoma were (40.9 ± 15.4) ng / ml, significantly higher than those in 63 healthy controls [(21.0 ± 7.3) ng / ml, P <0.0001] Patients with adenomas or polyps [(22.4 ± 8.5) ng / ml, P <0.0001]. Periostin ELISA kit has good sensitivity, specificity and accuracy, the future is expected to be used for the detection of clinical gastrointestinal tumors.