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目的:研究辽宁、吉林及朝鲜人参在免疫调节作用方面的药效差异。方法:检测三地人参对免疫抑制小鼠血清中TNF-α含量及脾细胞凋亡率的影响。结果:与正常空白对照组相比,免疫模型组的TNF-α含量明显下降(P<0.05);三地人参治疗组与正常空白对照组比较,TNF-α含量均无明显差异(P>0.05);三地人参治疗组之间相互比较,TNF-α含量均无明显差异(P>0.05)。与正常空白对照组相比,免疫模型组脾细胞凋亡率显著升高(P<0.005);三地人参治疗组与正常空白对照组比较,脾细胞凋亡率均无明显差异(P>0.05);三地人参治疗组与免疫模型组比较,免疫辽参治疗组脾细胞凋亡率明显低于免疫模型组(P<0.05);三地人参治疗组之间相互比较,脾细胞凋亡率均无明显差异(P>0.05)。结论:辽宁,吉林,朝鲜人参对免疫抑制小鼠均可促进TNF-α产生,抑制脾细胞凋亡,改善小鼠的免疫抑制状态,并且三地人参的免疫调节作用无明显差异。
OBJECTIVE: To study the difference in the immunomodulatory effects of ginseng in Liaoning, Jilin and North Korea. Methods: To detect the effect of ginseng on the content of TNF-α and the apoptosis rate of splenocytes in immunosuppressed mice. Results: Compared with the normal control group, the content of TNF-α in the immune model group was significantly decreased (P <0.05). There was no significant difference in TNF-α content between the three groups of ginseng treatment group and the normal control group (P> 0.05 ). There was no significant difference in TNF-α content between the three ginseng treatment groups (P> 0.05). Compared with the normal blank control group, the apoptotic rate of spleen cells in the immunized model group was significantly increased (P <0.005). Compared with the normal blank control group, the apoptotic rate of spleen cells in the three ginseng treatment groups was not significantly different (P> 0.05 ); Three ginseng treatment group compared with the immune model group, the immune cell apoptosis rate of LiaoShan treatment group was significantly lower than the immune model group (P <0.05); three ginseng treatment group compared with each other, spleen cell apoptosis rate No significant difference (P> 0.05). CONCLUSION: Ginseng in Liaoning, Jilin and North Korea can both promote the production of TNF-α, inhibit the apoptosis of spleen cells and improve the immunosuppressive status in immunosuppressed mice. There is no significant difference in immunoregulation between the three ginsengs.