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目的:运用SELDI蛋白质芯片技术分析体外培养的肝癌细胞株(HepG2)与正常肝细胞株(L02)蛋白质表达差异．方法:在体外培养HepG2和L02细胞株,收获细胞,将细胞用细胞裂解液裂解后,采用SELDI蛋白质芯片技术用IMAC3 及WCX2芯片检测HepG2、L02的蛋白质谱．结果:体外培养的肝癌细胞株与正常肝细胞株的蛋白质存在差异表达,IMAC3芯片共捕获61个蛋白,发现差异峰7个,与 L02细胞相比,其中3个差异蛋白在肝癌细胞中高表达,4个差异蛋白在肝癌细胞中低表达．WCX2芯片共捕获91个蛋白, 发现差异峰14个,其中3个差异蛋白在肝癌细胞中高表达,11 个差异蛋白在肝癌细胞中低表达．结论:SELDI蛋白芯片技术检测肝癌细胞株与正常肝细胞株蛋白质的差异表达方法简便,敏感性高,重复性好．
Objective: To analyze the difference of protein expression between HepG2 cells and normal liver cell lines (L02) by SELDI protein chip technology. Methods: HepG2 and L02 cell lines were cultured in vitro. Cells were harvested and lysed with cell lysate. The protein profiles of HepG2 and L02 were detected by SELDI protein chip technology with IMAC3 and WCX2 chips. Results: The proteins of hepatoma cell lines cultured in vitro and in normal liver cells were differentially expressed. IMAC3 chip captured a total of 61 proteins and found seven peaks. Compared with L02 cells, three of the differentially expressed proteins were highly expressed in hepatocellular carcinoma cells, Four differential proteins were low expressed in HCC cells. A total of 91 proteins were captured by WCX2 and 14 peaks were found. Among them, 3 differential proteins were highly expressed in hepatocellular carcinoma cells and 11 differential proteins were low expressed in hepatoma cells. Conclusion: SELDI protein chip technology for detection of liver cancer cell lines and normal liver cell protein differential expression method is simple, sensitive, reproducible.