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目的:建立一种酶标仪常规校准方法。方法:配制重铬酸钾溶液,用分光光度计450nm波长5mm比色杯比色结果与酶标仪主波长450nm参考波长630nm微孔板微孔内加样高度为5mm的比色结果进行比较,计算酶标仪的吸收度示值误差、示值稳定性、吸收度重复性、通道差异及相关性。结果:酶标仪吸收度示值误差、示值稳定性、吸收度重复性及通道差异均符合标准,且相关性良好,相关系数为0.999787。结论:该校准方法操作简单、结果可靠,可作为实验室常规校准酶标仪的方法。
Objective: To establish a standard calibration method of microplate reader. Methods: The potassium dichromate solution was prepared. The colorimetric results of the cuvette with a wavelength of 5mm at 450nm of the spectrophotometer were compared with the colorimetric results of the cuvette at a wavelength of 450nm and a reference wavelength of 630nm in the microplate at a wavelength of 450nm. Calculate the absorbance of the microplate reader indication error, indication stability, absorbance repeatability, channel differences and correlation. Results: The error of indication, the stability of indication, the repeatability of absorbance and the difference of channel were all accord with the standards, and the correlativity was good. The correlation coefficient was 0.999787. Conclusion: The calibration method is simple, reliable and can be used as a routine laboratory calibration microplate reader method.