论文部分内容阅读
目的研究组织病理学在非霍奇金淋巴瘤(NHL)侵犯骨髓中的诊断价值。方法骨髓活检标本观察组织形态,流式细胞学(FC)、免疫组化(IHC)进行免疫表型分析。结果 41例确诊为NHL病例中,B细胞淋巴瘤27例,T细胞淋巴瘤14例。侵犯骨髓的NHL分型:弥漫型15例(36.59%),混合型11例(26.83%),间质型9例(21.95%),结节型5例(12.20%),窦内型1例(2.44%)。FC可对37例NHL(90.24%)分型,其余4例需结合病史及骨髓IHC结果确定。15例同时行IHC检测,其中12例明确分型,其余3例(2例SLL/CLL,1例HCL)需结合FC进一步分型。1例DLBCL经FC仍不能分型需结合IHC确定。结论骨髓病理组织形态、免疫表型在NHL侵犯骨髓的诊断方面具有重要价值,流式细胞学与免疫组化在免疫分型方面互为补充。
Objective To investigate the diagnostic value of histopathology in the invasion of bone marrow by non-Hodgkin’s lymphoma (NHL). Methods Bone marrow biopsy specimens were observed for histomorphology, flow cytometry (FC) and immunohistochemistry (IHC) for immunophenotyping. Results Of the 41 cases diagnosed as NHL, there were 27 B-cell lymphoma and 14 T-cell lymphoma. NHL typing of infiltrating bone marrow: diffuse 15 cases (36.59%), mixed 11 cases (26.83%), interstitial 9 cases (21.95%), nodular 5 cases (12.20% (2.44%). FC can be 37 cases of NHL (90.24%) type, the remaining 4 cases need to be combined with history and bone marrow IHC results to determine. Fifteen patients underwent IHC at the same time, of which 12 were categorized correctly. The remaining 3 patients (2 patients with SLL / CLL and 1 patient with HCL) needed to be further typed with FC. One case of DLBCL still can not be typed by the combination of IHC to determine IHC. Conclusion Bone marrow histopathology and immunophenotype are of great value in the diagnosis of NHL invaded bone marrow. Flow cytometry and immunohistochemistry complement each other in immunophenotyping.