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利用北京同步辐射装置(BSRF)4W1A光束线提供的硬X射线,以类同轴全息法、衍射增强法(Diffractionenhancedimaging,DEI),对兔正常骨、骨缺损;小鼠正常肺、肺癌;大鼠正常肝、肝纤维化、肝硬化等固定样品进行了同步辐射硬X射线相衬成像观察。在此基础上,成像组织及空白对照组织均按常规方法制片后进行HE染色,在光学显微镜下观察组织细胞形态结构;采用免疫组织化学技术检测其相关抗原Ⅷ、S-100、FN、泛素的表达,并根据抗原表达强度进行半定量分析。结果显示,成像组与空白对照组的骨、软骨、肝、肺组织染色均匀,结构清晰,无细胞损伤;两组骨、肝、肺相关抗原表达无明显差异。表明在以同步辐射硬X射线相衬成像方法观察固定组织的过程中,不会对骨、软骨、肝、肺组织的形态结构及一些相关抗原表达造成损伤和影响。
Using normal X-rays provided by Beijing Synchrotron Radiation Facility (BSRF) 4W1A beamline, normal rabbits and bones were sacrificed by coaxial holography and diffraction-enhanced diffraction (DEI) Normal liver, liver fibrosis, liver cirrhosis and other fixed samples were observed by synchrotron radiation X-ray phase contrast imaging. On this basis, the imaging tissues and blank control tissues were prepared by conventional methods after HE staining, the morphological structure of the cells was observed under an optical microscope; immunohistochemistry was used to detect the relative antigen Ⅷ, S-100, FN, The expression of the protein was determined by semi-quantitative analysis. The results showed that the bone, cartilage, liver and lung tissue of the imaging group and the blank control group were stained uniformly, with clear structure and no cell damage. There was no significant difference between the two groups in the expression of the related antigens of bone, liver and lung. It is indicated that the morphological structure of bone, cartilage, liver and lung tissue and the expression of some related antigens will not be damaged and affected during the observation of fixed tissue by synchrotron radiation hard X-ray phase contrast imaging.