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目的:研究地锦草对糖尿病小鼠胰岛β细胞氧化应激和凋亡的影响。方法:尾静脉注射四氧嘧啶溶液(80 mg·kg-1)复制糖尿病小鼠模型。将造模成功的小鼠随机分为5组:模型组,阳性对照组(盐酸二甲双胍悬浊液25 mg·kg-1·d-1),地锦草低、中、高剂量组(59,118,236 mg·kg-1·d-1),连续给药14 d后,观察其血糖、胰腺组织超氧化物歧化酶(SOD),谷胱甘肽过氧化物酶(GSH-Px),丙二醛(MDA)水平的变化,采用末端脱氧核苷酸转移酶介导的d UTP缺口末端标记法(TUNEL法)检测胰岛β细胞的凋亡情况。结果:与模型组比较,地锦草各剂量组小鼠体重下降不明显(P<0.05);与给药前及模型组比较,地锦草高剂量组和二甲双胍组血糖均下降(P<0.05);与模型组比较,地锦草高剂量组和二甲双胍组胰腺组织中SOD和GSH-Px活性均升高(P<0.05),MDA的含量降低(P<0.05),胰岛β细胞凋亡指数降低(P<0.05)。结论:地锦草可明显降低糖尿病小鼠的血糖,其机制可能与增强糖尿病小鼠的抗氧化能力,减弱氧化应激对糖尿病小鼠胰岛β细胞的损伤,抑制胰岛β细胞的凋亡有关。
Objective: To study the effect of Caoshen grass on oxidative stress and apoptosis of pancreatic β cells in diabetic mice. Methods: Allo-pyrimidine solution (80 mg · kg-1) was injected into caudal vein to duplicate the diabetic mouse model. The model mice were randomly divided into 5 groups: model group, positive control group (metformin hydrochloride suspension 25 mg · kg -1 · d -1), Radix et Rhizoma Polygonatum low, medium and high dose groups (59, 118, 236 mg Kg-1 · d-1). After continuous administration for 14 days, the blood glucose, the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) MDA) levels were detected by terminal deoxynucleotidyl transferase mediated d UTP nick end labeling (TUNEL method) detection of pancreatic β-cell apoptosis. Results: Compared with model group, the body weight of mice in each dose group was not decreased significantly (P <0.05). Compared with the model group, the blood glucose of high dose group and metformin group decreased (P <0.05 ). Compared with model group, the activity of SOD and GSH-Px in pancreatic tissue of high dose of gentian and metformin group were increased (P <0.05), the content of MDA was decreased (P <0.05) Decreased (P <0.05). Conclusion: Echinacea purpurea can significantly reduce the blood sugar in diabetic mice, and its mechanism may be related to enhancing the anti-oxidation ability of diabetic mice, decreasing the oxidative stress on the islet β-cell damage and inhibiting the apoptosis of pancreatic β-cells in diabetic mice.