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目的分析临床分离金黄色葡萄球菌(Staphylococcus aureus,S.aureus)的耐药性,毒性和生物膜(Bacterial biofilm,BF)形成能力,并进一步探究三者之间的关系,为临床预防和治疗S.aureus感染提供理论依据。方法临床分离的122株S.aureus经全自动微生物分析仪鉴定及药物敏感性检测,采用结晶紫染色和刚果红平板法分析生物膜形成能力;通过对其溶血素、胞外蛋白酶和脂肪酶的检测评估其毒性。结果 122株S.aureus甲氧西林耐药株(MRSA)分离率达到将近70%,且表现出多重耐药现象;31.97%能产生溶血素,29.51%能产生胞外蛋白酶,54.10%能产生胞外脂肪酶;约40%的菌株有一定的生物膜形成能力,且MRSA的生物膜形成能力强于甲氧西林敏感株(MSSA),毒性弱于MSSA。结论 S.aureus感染引起的不同临床疾病主要与其产生的毒素和耐药性有关,而细菌的生物膜对于其毒性和耐药性同样有很重要的影响。
Objective To analyze the drug resistance and toxicity of Staphylococcus aureus (S. aureus) and the formation of biofilm (BF) in clinical isolates, and to further explore the relationship between the three for the clinical prevention and treatment of S Aureus infection provides a theoretical basis. Methods 122 strains of S. aureus clinically isolated were identified by automatic microbiological analyzer and tested for drug susceptibility. The biofilm formation ability was analyzed by crystal violet staining and Congo red plate method. The hemolysin, extracellular protease and lipase Testing to assess its toxicity. Results The isolation rates of 122 MRSA strains of S. aureus reached nearly 70%, and showed multi-drug resistance. 31.97% could produce hemolysin, 29.51% could produce extracellular protease and 54.10% About 40% of the strains had some biofilm-forming ability. MRSA had stronger biofilm-forming ability than methicillin-sensitive strain (MSSA) and weaker than MSSA. Conclusions Different clinical diseases caused by S. aureus infection are mainly related to the toxins and drug resistance they produce. However, bacterial biofilms also have an important impact on their toxicity and drug resistance.