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为探讨日粮粗蛋白(crude protein,CP)水平对生长育肥猪肠道微生物胞内酶活性的影响,试验分别选择体质量分别为(30±2.54),(60±3.18)kg的杜×长×大三元杂交猪各18头,均采用单因子完全随机分组设计分配到CP为12%,15%,18%和10%,13%,16%的3组日粮中,每组6个重复,每个重复1头猪。预试期7d,正试期30d。在正饲期31d,每组随机挑选6头进行屠宰,采集各肠段食糜和粪便样品,研究日粮CP水平对生长育肥猪肠道微生物胞内酶活性的影响。结果表明,生长育肥猪肠道微生物酶活力均具有肠段特异性;日粮CP水平能显著影响猪肠道微生物硝酸还原酶(P<0.05)、谷氨酰胺合成酶(P<0.05)、谷草转氨酶(P<0.05)、谷丙转氨酶(P<0.05)、乳酸脱氢酶(P<0.05)、尿素酶(P<0.05)及蛋白酶(P<0.05)水平;日粮CP水平对肠道微生物的蛋氨酸、谷氨酸、精氨酸、酪氨酸、色氨酸脱羧酶活力影响显著(P<0.05);日粮CP水平显著影响肠道微生物酶的活力,是肠道微生物对氨基酸差异性降解和动物对蛋白质利用率低的原因之一。
In order to investigate the effects of dietary crude protein (CP) levels on the intracellular enzyme activities of gut micro-organisms in growing fattening pigs, the body weight of each group was (30 ± 2.54), (60 ± 3.18) kg × large ternary crossbred pigs were randomly divided into three groups with 12%, 15%, 18% and 10%, 13% and 16% CP respectively Repeat, one pig per repeat. Pre-trial period 7d, the trial period 30d. In the feeding period of 31d, each group of six randomly selected slaughtering, collecting each segment of the digestive tract and stool samples to study dietary CP level of growth and finishing pig intestine microbial intracellular enzyme activity. The results showed that the intestine-specific microbial enzyme activity of intestine in growing-finishing pigs was intestinal-specific. Dietary CP level could significantly affect the intestinal microbial nitrate reductase (P <0.05), glutamine synthetase (P <0.05) (P <0.05), ALT (P <0.05), lactate dehydrogenase (P <0.05), urease (P <0.05) and protease (P < (P <0.05). The dietary CP level significantly affected the activity of intestinal microbial enzymes, which is the effect of intestinal microbial on amino acid diversity Degradation and animal utilization of protein is one of the reasons.