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[目的]研究胃安宁颗粒含药血清体外对人胃癌MGC-803细胞株增殖、凋亡的影响。[方法]采用MTT比色法检测不同剂量胃安宁颗粒含药血清作用12h、24h、48h后对MGC-803细胞生长增殖的影响,流式细胞仪检测细胞凋亡率。[结果]随着胃安宁颗粒含药血清浓度逐渐增加,细胞增殖率逐渐降低,细胞抑制率、凋亡率逐渐升高,且以早期凋亡为主。[结论]胃安宁颗粒能够抑制人胃癌MGC-803细胞的增殖,对MGC-803细胞有诱导其早期凋亡作用。
[Objective] To investigate the effect of Weianning granule-containing serum on proliferation and apoptosis of human gastric cancer cell line MGC-803 in vitro. [Method] MTT colorimetric assay was used to detect the effect of different doses of Weianning granule on the growth and proliferation of MGC-803 cells after 12h, 24h and 48h treatment. The apoptosis rate was detected by flow cytometry. [Result] With the increasing concentration of drug-containing serum of Weianning Granules, the cell proliferation rate gradually decreased, the cell inhibition rate and apoptosis rate gradually increased, and mainly the early apoptosis. [Conclusion] Weianning Granule can inhibit the proliferation of human gastric cancer cell line MGC-803 and induce the early apoptosis of MGC-803 cell line.