To gain more precise information about molecular genetic variation for wild populations of Blumeria graminis f. sp. tritici from Qinghai Province, China, 38 single-colony isolates were purified from samples collected from Haidong District, Xining City and Hainan Tibetan Autonomous Prefecture in 2010. The virulence of 21 isolates among them was tested at seedling stage on 34 wheat cultivars(lines) carrying known powdery mildew(Pm) resistant genes. The results showed that V1 a, V3 a, V3 c, V3 e, V5 a, V6, V7, V8 and V19 had high virulence frequencies(>75%), indicating a wide distribution; and V1 c, V5 b, V12, V13, V16, V21, VXBD, V2+6, V2+Mld and V4+8, with less distribution, appeared to be lower in frequencies(0-20%). The Nei’s gene diversity(H), Shannon’s information index(I) and the percentage of polymorphic loci(P) were 0.23, 0.35 and 67.65%, respectively, which revealed a virulent diversity. The results from single nucleotide polymorphisms(SNPs) of 38 isolates showed that three housekeeping genes were found to contain a total of 9 SNP sites. 10 haplotypes(H1-H10) were inferred from the concatenated sequences, with 1 haplotype(H1) comprising of over 55% of Qinghai population. Phylogenic analysis did not show obvious geographical subdivision between the isolates. A multilocus haplotype network presented a radial structure, with H1 in the central as an inferred ancestor. Using analysis of molecular variance(AMOVA), we found 1.63% of the total variation was among populations and 98.37% within populations, with a low fixations index(FST=0.01634, P<0.05). This revealed a relatively high genetic diversity but a low genetic divergence in Qinghai population. Moreover, the molecular data on gene flow(Nm=6.32) confirmed the migration of pathogen populations among areas in Qinghai Province. To gain more precise information about molecular genetic variation for wild populations of Blumeria graminis f. Sp. Tritici from Qinghai Province, China, 38 single-colony isolates were purified from samples collected from Haidong District, Xining City and Hainan Tibetan Autonomous Prefecture in 2010. The virulence of 21 isolates among them was tested at seedling stage on 34 wheat cultivars (lines) carrying known powdery mildew (Pm) resistant genes. The results showed that V1a, V3a, V3c, V3e, V5a, V6, V7, V8 and V19 had high virulence frequencies (> 75%) indicating a wide distribution; and V1 c, V5 b, V12, V13, V16, V21, VXBD, V2 + 6, V2 + Mld and V4 + 8, with The Nei’s gene diversity (H), Shannon’s information index (I) and the percentage of polymorphic loci (P) were 0.23, 0.35 and 67.65%, respectively, which were lower than frequencies (0-20% revealed a virulent diversity. The results from single nucleotide polymorphisms (SNPs) of 38 isolates showed that three hous Phylogenic analysis did not show obvious geographical subdivision (H1-H10) were inferred from the concatenated sequences, with 1 haplotype (H1) comprising over 55% of Qinghai population. Between the isolates. A multilocus haplotype network presented a radial structure, with H1 in the central as an inferred an actast. Using analysis of molecular variance (AMOVA), we found 1.63% of the total variation was among populations and 98.37% within populations, with A low fixations index (FST = 0.01634, P <0.05). This revealed a relatively high genetic diversity but a low genetic divergence in Qinghai population. Moreover, the molecular data on gene flow (Nm = 6.32) confirmed the migration of pathogen groups among areas in Qinghai Province.