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为了丰富马铃薯分子标记,定位青枯病抗性基因位点,利用具有抗青枯病遗传背景的二倍体马铃薯亲本USW5337.3(C)和772102.37(E)及其123份杂交一代无性系,进行了马铃薯SSR遗传图谱的构建.根据马铃薯全基因组序列设计了864对SSR引物,其中187对在亲本间表现出差异,135对(72.2％)能够在杂交一代中扩增出清晰的条带,最终构建了一张由12个连锁群组成,包含135个SSR标记的马铃薯分子标记遗传图谱.12个连锁群总长度为948.4 cM,标记间平均间距7.03 cM,含有5个标记偏分离区域.不仅为马铃薯饱和分子遗传图谱的构建提供新的SSR标记,对于马铃薯抗青枯病QTL定位、基因克隆和分子标记辅助选择也具有重要的参考意义.“,”In order to enrich molecular markers and position the bacterial wilt resistant gene loci in potato,an SSR-based genetic linkage map was constructed from a diploid population consisting of 123 individuals derived from a cross between two potato diploid clones ( C and E) which harborer resistance to Ralstonia solanacearum in this study. A total of 864 simple sequence repeat primer pairs were designed according to the potato genome sequence and used to screen parents C and E for polymorphisms. Of 187 polymorphic loci,72. 2％ showed clear amplifiable and scorable bands in the F1 population. Then a total of 135 locus were mapped to 12 linkage groups,of which the cumulative length was 948. 4 cM,with an average marker interval of 7. 03 cM,and 5 segregation distortion regions in three linkage groups. This study provided a set of new SSR markers for genetic map construction and increased satu-ration of map in potato. The relatively high density map may provide a useful reference for further identification, mapping of quantitative trait loci ( QTL) and cloning of potato bacterial wilt resistance genes and marker-assisted breeding.