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目的 探讨依托咪酯、咪唑安定对离体兔肺动脉的直接效应以及内皮的完整性对其效应的影响。方法 制备内皮完整与去内皮兔离体肺动脉环 ,观察两种药物累积剂量 0 .0 3mmol/ L、0 .1mmol/ L、0 .3mm ol/ L、1.0 m mol/ L、3.0 mm ol/ L 对肺动脉的直接作用及对去甲肾上腺素 (NE)预收缩肺动脉的作用 ;两种药物 1m mol/ L对 NE累积剂量量 -效曲线的影响。结果 两种药物累积剂量对张力正常肺动脉环无明显影响 ;对 NE预收缩肺动脉环有剂量依赖性的舒张作用 ,对完整内皮与去内皮动脉环舒张作用无明显差异 (P>0 .0 5 ) ;两种药物 1m mol/ L 可使 NE累积剂量量 -效曲线呈非平行性的右移 ,高剂量 NE〔(10 - 5～ 10 - 3) mol/ L〕时收缩幅度较 NE对照组明显降低 (P<0 .0 5 )。结论 依托咪酯、咪唑安定对张力正常兔离体肺动脉环无直接作用 ;对 NE预收缩肺动脉的舒张作用呈非内皮依赖性 ,对 NE的肺动脉收缩效应表现非竞争性抑制作用。
Objective To investigate the effects of etomidate and midazolam on the pulmonary artery in vitro and the integrity of endothelial cells. Methods Pulmonary arterial rings with intact endothelium and endothelium were isolated and the cumulative doses of two drugs were observed. The cumulative doses of the two drugs were 0.3 mmol / L, 0.1 mmol / L, 0.3 mmol / L, 1.0 mmol / L and 3.0 mmol / L The direct effect on the pulmonary artery and the effect of norepinephrine (NE) precontracted pulmonary artery; the effect of two drugs 1m mol / L on NE cumulative dose-response curve. Results The cumulative doses of the two drugs had no significant effect on the normal pulmonary artery rings. The diastolic effect on NE precontracted pulmonary rings was dose-dependent and there was no significant difference between the intact endothelium and the endothelium (P> 0.05) ; The two drugs at a dose of 1 m mol / L could shift the dose-response curve of NE dose-dependently to the right shift and the contractile amplitude at NE (10 - 5 ~ 10 -3 mol / L) Decreased (P <0.05). Conclusions Etomidate and midazolam have no direct effect on the isolated pulmonary artery rings of normal tension rats. The diastolic effect on NE pre-contracted pulmonary artery is non-endothelium-dependent and exerts a noncompetitive inhibitory effect on NE pulmonary contractions.