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为了发展南京地区中药材栽培产业,引进和推广桔梗优良品系,对我们前期研究获得的同源四倍体桔梗优良品系进行了生理、生化指标的测定和评价。通过测定并比较2年生、3年生桔梗2倍体和4倍体品系的4项主要生理、生化指标,为桔梗多倍体优良品系的选育,评价提供依据。试验结果表明:无论2年生、3年生的桔梗植株,同源四倍体桔梗品系的叶绿素含量、APX、POD和SOD的生理生化指标均比对应的2倍体明显提高,同源四倍体桔梗品系生长代谢更旺盛,预计有更好的生物产量、抗逆性和代谢产物积累活性。该实验建立的4倍体桔梗的生理生化指标的测定和评价方法,对其他中药材优良品种的选育和评价具有重要的参考价值。
In order to develop the cultivated industry of Chinese medicinal materials in Nanjing area and introduce and popularize the excellent lines of Campanulaceae, the physiological and biochemical indexes of the excellent tetraploid platycodon lines obtained in our previous study were determined and evaluated. By measuring and comparing 4 major physiological and biochemical indexes of 2-year-old and 3-year-old Platycodon diploid and 4-year-old lines, the results provided the basis for selection and evaluation of good-breed polyploid lines. The results showed that the chlorophyll content, physiological and biochemical indexes of APX, POD and SOD in two-year-old and three-year-old Campanulaceae cultivars and the autotetraploid Campanulaceae were significantly higher than those in the corresponding diploid. The autotetraploid Strain growth and metabolism more robust, expected to have better biological yield, resistance and accumulation of metabolites activity. The determination and evaluation method of physiological and biochemical indexes of 4-fold Platycodon grandiflorum established in this experiment have important reference value for the breeding and evaluation of other excellent varieties of Chinese herbal medicines.