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采用反相高效液相色谱法,Ultimate~C_(18)色谱柱,对红参中9种人参皂苷类成分进行了具有高分离度和高色谱峰纯度的同步色谱分离。采用“一测多评”中药质量评价模式,经一系列方法学考察,以人参皂苷Rb_1为内参物,建立了人参皂苷Rg_1,Re,Rf,Rb_1,Rc,Rb_2,Rb_3,Rd和20(S)-人参皂苷Rg3与人参皂苷Rb1间的相对校正因子与相对保留值,在红参对照图谱的辅助定位下,最终实现了仅采用人参皂苷Rb1一个中药对照品对红参中上述9种人参皂苷类成分的同步质量控制。
Using reversed-phase high-performance liquid chromatography, Ultimate ~ C_ (18) column, the chromatographic separation of nine ginsenosides in red ginseng with high resolution and high chromatographic purity was carried out. The ginsenosides Rb_1, Re, Rf, Rb_1, Rc, Rb_2, Rb_3, Rd and 20 were established by a series of methodological studies using the " (S) - ginsenoside Rg3 and ginsenoside Rb1 between the relative correction factor and the relative retention value in red ginseng control map assisted positioning, the ultimate realization of the only use of ginsenoside Rb1 a Chinese medicine reference substance of red ginseng in the above-mentioned 9 Synchronous quality control of ginsenosides.