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60只雄性大鼠,20只为对照组,余用链脲佐菌素(STZ)制备2型糖尿病大鼠模型。成模后,随机分成两组,糖尿病非药物干预组及阿托伐他汀干预组。干预组予阿托伐他汀(2mg/kg/day)灌胃,对照组及糖尿病非药物干预组予等量饮用水灌胃。3个月处死大鼠。取一部分心肌组织抽提RNA,另一部分心肌组织固定后做免疫组织化学观察。应用RT-PCR方法扩增NF-κB基因,比较3组大鼠的NF-κB在基因水平上的表达差异,RT-PCR方法检测心肌组织中NF-κB的mRNA表达水平,免疫组织化检测其蛋白表达。结果:糖尿病大鼠心肌组织中NF-κB在mRNA表达及蛋白表达比正常大鼠明显增高(P<0.05),给药3月NF-κB的mRNA表达及蛋白表达比糖尿病非药物干预组明显减少(P<0.05)。结论:阿托伐他汀能降低糖尿病大鼠心肌组织中NF-κB mRNA及NF-κB蛋白质的表达,减缓心肌组织病变进展。
Sixty male rats and twenty mice were used as the control group. Rats in type 2 diabetes were treated with streptozotocin (STZ). After modeling, they were randomly divided into two groups: diabetic non-drug intervention group and atorvastatin intervention group. The intervention group was given atorvastatin (2mg / kg / day) orally, and the control group and diabetic non-drug intervention group were given the same amount of drinking water. Rats were sacrificed at 3 months. Take part of the myocardial tissue RNA extraction, the other part of the myocardial tissue fixed immunohistochemical observation. The gene of NF-κB was amplified by RT-PCR. The mRNA expression of NF-κB in myocardium was detected by RT-PCR. The expression of NF-κB in myocardium was detected by immunohistochemistry Protein. Results: The mRNA and protein expression of NF-κB in myocardium of diabetic rats increased significantly (P <0.05), and the mRNA and protein expression of NF-κB in diabetic rats decreased significantly compared with non-diabetic diabetic rats (P <0.05). Conclusions Atorvastatin can decrease the expression of NF-κB mRNA and NF-κB protein in myocardium of diabetic rats and alleviate the progression of myocardial lesions.