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在薄叶黄芩成分分离的过程中,用反相HPLC分离葡萄糖醛酸黄酮甙,包括柱和溶媒系统等的条件选择:并发现当羟基和葡萄糖醛酸基的取代位置不同时,其保留时间的变化规律(简称构保关系)。例如,当羟基从4位变化到2位时,保留时间增大:2′,6,8位羟基的存在,保留时间依次增大;而当葡萄糖醛酸基从7位变化到8位时,保留时间相应变小。有人提出在相同位置上都存在有羟基的黄酮类化合物在一起时,是用HPLC不能得到很好分离的“临界群”,如易与羰基形成内氢键的5位羟基。本文方法解决了这一问题,成功地分离到的7个葡萄糖醛酸黄酮甙,5位都存在有羟基。本文还介绍了两种不同型号的ODS柱对分离葡萄糖醛酸黄酮甙的效果。
Separation of glucosinolate glycosides by reversed-phase HPLC, including the column and solvent systems, during the separation of the components of the Astragalus membranaceus and the retention time of the glucuronyl groups when they are substituted Law of change (referred to as the formation of protection relationship). For example, when the hydroxyl group changes from 4-position to 2-position, the retention time increases: the presence of hydroxyl groups at the 2′, 6 and 8 positions increases, and the retention time increases sequentially; when the glucuronic acid group changes from 7-position to 8-position, The retention time becomes smaller accordingly. It has been proposed that hydroxy-containing flavonoids are present together at the same position and are not a good “critical group” that can be separated by HPLC, such as the 5-position hydroxyl group that easily forms an internal hydrogen bond with the carbonyl group. This method solves this problem. The 7 glucuronoside glycosides successfully isolated have hydroxyl groups at the 5-position. This article also describes the effect of two different types of ODS columns on the separation of glucuronide.