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目的 建立同时测定金银花不同种(忍冬、山银花、红腺忍冬、灰毡毛忍冬、细毡毛忍冬)中芦丁、金丝桃苷、木犀草素7 O β D 半乳糖苷、忍冬苷、苜蓿苷、金圣草素 7 O 新橙皮糖苷、苜蓿素 7 O 新橙皮糖苷、槲皮素8种黄 酮的HPLC分析方法。方法 色谱柱为AglientZorbax80A,Extend C18(250mm×4.6mmID,5μm)。梯度洗脱,流动 相A为1.2%THF,0.5%HAc;B为甲醇 乙腈(40∶60)。流速1.0mL·min-1,检测波长355nm。结果 线性范围内 8个黄酮化合物的标准曲线呈良好的线性关系,平均加样回收率均在97%-102%。所有化合物的精密度和重复性 的RSD均<3%,定量限(S/N=10)低于6.0ng。结论 该分析方法简单、灵敏、准确、重复性好,可同时分析金银花 中的黄酮类成分。
Objective To establish a method for simultaneous determination of rutin, hyperoside, luteolin 7 O β D galactoside, and honeysuckle in different species of honeysuckle (Lonicera japonica, Cinnamomum villosum, Lonicera magna, Lonicera macranthoides, Lonicera macranthoides). HPLC analysis methods of 8 kinds of flavonoids, quercitrin, saponin 7O, neocortical anthroquinone 7 O neo-total glucoside, quercetin. Methods The column was Aglient Zorbax 80A, Extend C18 (250 mm×4.6 mm ID, 5 μm). Gradient elution, mobile phase A was 1.2% THF, 0.5% HAc; B was methanol acetonitrile (40:60). The flow rate was 1.0 mL·min-1 and the detection wavelength was 355 nm. Results The calibration curve of the eight flavonoids in the linear range showed a good linear relationship. The average recovery rate was between 97% and 102%. The precision and repeatability of all compounds were <3% RSD, and the limit of quantitation (S/N=10) was less than 6.0 ng. Conclusion The analytical method is simple, sensitive, accurate, and reproducible. It can simultaneously analyze flavonoids in honeysuckle.