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目的观察活血通腑方对肠粘连的治疗效果,探讨其对肠粘连防治作用的机理。方法取SD雄性大鼠60只,随机分为6组:对照组、模型组、活血通腑方高、中、低剂量组、地塞米松磷酸钠阳性对照组。除对照组外,其余各组大鼠均制备肠粘连模型。对照组和模型组腹腔注射生理盐水,地塞米松组腹腔注射地塞米松磷酸钠(10mg/kg),活血通腑方低、中、高剂量组灌胃给予活血通腑方(0.65、1.3、2.6g/100g),连续给药1周。各组于术后第8天取血,ELISA法测定血清IL-6、IL-1β和TNF?α含量;取粘连组织,匀浆后试剂盒检测TGF-β1、CTGF的含量,同时记录大鼠肠粘连级别。RT-PCR检测粘连组织中FN mRNA的表达。Western blot检测p-Smad3的蛋白水平。结果模型组血清IL-1β和TNF?α含量增高,FN mRNA的表达增加,TGF-β1、CTGF含量增高。与模型组相比,活血通腑方低、中、高剂量组的肠粘连程度明显减轻,血清IL-1β和TNF?α及粘连组织中TGF-β1和CTGF含量明显下降(P<0.05~0.01),FN mRNA和p-Smad3表达降低(P<0.05~0.01)。结论活血通腑方可减轻肠粘连的程度,对肠粘连具有防治作用。
Objective To observe the therapeutic effect of Huoxue Tongfu Decoction on intestinal adhesion, and to explore its mechanism of prevention and treatment of intestinal adhesion. Methods Sixty SD male rats were randomly divided into 6 groups: control group, model group, high, medium and low dose Huoxuetongfukang group, and dexamethasone sodium phosphate positive control group. In addition to the control group, the other groups of rats were prepared intestinal adhesion model. Rats in control group and model group were intraperitoneally injected with normal saline. Dexamethasone group was given intraperitoneal injection of dexamethasone sodium phosphate (10mg / kg) 2.6 g / 100 g) for 1 week. The levels of IL-6, IL-1β and TNF-α in serum were determined by ELISA method on the 8th postoperative day. The contents of TGF-β1 and CTGF in the tissue were detected by ELISA. Intestinal adhesion level. The mRNA expression of FN in adhesion tissue was detected by RT-PCR. Western blot detection of p-Smad3 protein levels. Results The levels of serum IL-1β and TNF-α in model group were increased, the expression of FN mRNA and the content of TGF-β1 and CTGF were increased. Compared with the model group, the intestinal adhesion of Huoxuetongfukang low, medium and high dose groups was significantly reduced, and the levels of IL-1β, TNFα and the content of TGF-β1 and CTGF in the adhesion tissue were significantly decreased (P <0.05-0.01 ), FN mRNA and p-Smad3 expression decreased (P <0.05 ~ 0.01). Conclusions Huoxue Tongfu Decoction can relieve the degree of intestinal adhesion, which has a preventive and therapeutic effect on intestinal adhesion.