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玉米(Zea mays L.)是世界第三大粮食作物,也是重要的能源、饲料植物.本研究以玉米毛状根再生植株为材料,吉单35自交系为对照,设置三种氮供应条件,探究氮高效利用的玉米毛状根再生植株光合作用特性.结果显示,玉米毛状根再生植株的净光合速率(Pn)、气孔导度(Gs)和蒸腾速率(E)均高于对照组玉米,胞间CO2浓度(Ci)低于对照.此外,在低氮供应下,毛状根再生植株的净光合速率显著高于对照,说明玉米毛状根再生植株能在养分供应不足的情况下高效地利用氮素.本研究为培育氮高效利用的玉米新品种奠定基础.“,”Maize is the third largest food crop in the world as well as important feed and energy plant.In this research,maize regenerative plants from hairy root cultures were took as plant materials,and Jidan 35 was took as control group.Photosynthetic characteristics of nitrogen efficient maize regenerative plants from hairy root cultures were analyzed under three nitrogen supply levels.Results showed that the net photosynthetic rate (Pn),stomatal conductance (Gs),and transpiration rate (E) of maize regenerative plants from hairy root cultures were all higher than control,whereas intercellular CO2 concentration (Ci) was lower than control.Moreover,the net photosynthetic rate of maize regenerative plants from hairy root cultures was significant higher than control under low nitrogen supply.It indicated that maize regenerative plants from hairy root cultures had high nitrogen use efficiency when nutrient supply was not sufficient.This study laid the foundation for breeding new maize varieties with high nitrogen use efficiency.