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目的:建立高效液相色谱-串联质谱法检测油酸和亚油酸含量的方法,从而对鸦胆子油自微乳给药系统中鸦胆子油的肠吸收进行研究。方法:以甲醇-水(95:5 v/v)为流动相,流速为0.4 mL/min,柱温为35℃作为高效液相色谱的检测条件。利用大鼠小肠膜建立体外药物扩散体系研究鸦胆子油的肠吸收特性。结果:油酸和亚油酸的保留时间分别为10.46±0.02和8.55±0.01 min,线性范围分别为0.50~50.0 ng/mL和5.06~101.2 ng/mL,平均绝对回收率分别为97.49±3.11%和105.76±3.13%。日间和日内精密度都小于5%。在肠吸收实验中,鸦胆子油自微乳体系中测得油酸和亚油酸含量是单独给予鸦胆子油测得量的2.8和4.1倍。结论:该方法高效、灵敏、选择性高,可以用于鸦胆子油及鸦胆子油自微乳肠吸收中油酸和亚油酸的质量测定。
OBJECTIVE: To establish a method for the determination of oleic acid and linoleic acid by high performance liquid chromatography-tandem mass spectrometry, and to study the intestinal absorption of Brucea javanica oil in Brucea javanica oil self-microemulsion delivery system. Methods: The mobile phase was methanol-water (95: 5 v / v), the flow rate was 0.4 mL / min and the column temperature was 35 ℃. Study on Intestinal Absorption of Brucea Javanica Oil Using In vitro Drug Diffusion System of Small Intestine Membrane in Rats. Results: The retention times of oleic acid and linoleic acid were 10.46 ± 0.02 and 8.55 ± 0.01 min, respectively. The linear range was 0.50 ~ 50.0 ng / mL and 5.06 ~ 101.2 ng / mL, with the average absolute recoveries of 97.49 ± 3.11% And 105.76 ± 3.13%. Precision is less than 5% during the day and the day. In intestinal absorption experiments, Brucea javanica oil microemulsion system measured oleic acid and linoleic acid content alone was Brucea oil measured 2.8 and 4.1 times. Conclusion: The method is efficient, sensitive and selective. It can be used to determine the content of oleic acid and linoleic acid in the micro-intestinal absorption of Brucea javanica oil and Brucea javanica oil.