【摘 要】
：
分别以5-碘-2’-脱氧尿苷、5-碘-2’-脱氧胞苷、7-去氮-7-碘-2’-脱氧腺苷及7-去氮-7-碘-2’-脱氧鸟苷为原料,以二硫键为可裂解连接单元,通过多步反应合成了四色荧光标记不同
【机 构】
：
上海交通大学系统生物医学研究院,系统生物医学教育部重点实验室,华东理工大学化学与分子工程学院,先进材料重点实验室和精细化工研究所,上海交通大学生物医学工程学院,Bio-ID研究中心,北京师范大学化学学
论文部分内容阅读
分别以5-碘-2’-脱氧尿苷、5-碘-2’-脱氧胞苷、7-去氮-7-碘-2’-脱氧腺苷及7-去氮-7-碘-2’-脱氧鸟苷为原料,以二硫键为可裂解连接单元,通过多步反应合成了四色荧光标记不同碱基的脱氧核糖核苷酸;研究了该类荧光标记核苷酸作为可逆终端在DNA合成测序中的应用.所得产物结构经1H NMR,31P NMR及HRMS表征,并对其进行了DNA高通量测序的测试.结果表明,该类荧光标记核苷酸作为DNA合成测序的可逆终端能够满足高通量测序的生化反应要求,具有较好的应用前景.
5-iodo-2’-deoxycytidine, 7-deaza-7-iodo-2’-deoxyadenosine and 7-deaza-7-iodo-2 ’- deoxyguanosine as the starting material, the disulfide bond as a cleavable linker unit, by four-step reaction was synthesized four-color fluorescently labeled different bases of deoxyribonucleotides; study of such fluorescently labeled nucleotide as a reversible terminal In the DNA sequencing.The structure of the obtained product was characterized by 1H NMR, 31P NMR and HRMS and tested by DNA high-throughput sequencing.The results showed that these fluorescently labeled nucleotides were reversible as DNA sequencing The terminal can meet the biochemical reaction requirements of high-throughput sequencing and has a good application prospect.
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