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棉籽是主要的植物蛋白资源,但棉仁的紫色腺体中含有毒成分棉子酚(半数致死量LD_(50)=2570毫克/千克),故长期未能合理利用。近年发现,棉子酚有抗癌作用,为男性避孕药,还用于镇咳。目前各产棉区正研究棉籽的去毒与综合利用,棉子酚测定成为例行性工作。以往测定棉子酚,通常用苯胺或三氯化锑比色法。三氯化锑的氯仿溶液忌水,即使遇微量水也产生沉淀,且灵敏度较差;苯胺易氧化呈橙色,须重新精馏。本文介绍棉子酚的紫外吸收法测定。条件试验1.不同溶剂中棉子酚的紫外光谱
Cottonseed is the main plant protein resource, but cottonseed purple gland contains the toxic component gossypol (LD 50 = 2570 mg / kg), so it has not been properly used for a long time. In recent years found that gossypol has anti-cancer effects for male contraceptives, but also for antitussive. At present, cotton production areas are studying the detoxification and comprehensive utilization of cottonseed, gossypol determination as a routine work. Gossypol was measured in the past, usually with aniline or antimony trichloride colorimetry. Antimony trichloride in chloroform solution bogey, even in case of trace amounts of water also produce precipitation, and the sensitivity is poor; Aniline easily oxidized orange, to be re-distillation. This article describes the determination of gossypol UV absorption method. Condition Test 1. UV spectroscopy of gossypol in different solvents