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目的 探讨铁路危险品货运站空气颗粒污染物对小鼠外周血网织红细胞微核的诱导作用。方法 选用雄性BAL B/c小鼠随机分为实验组和对照组 ,空气颗粒污染物的丙酮提取物按 2 0、4 0、80、16 0 mg/kg的剂量 ,以腹腔注射方式一次性染毒。同时进行低剂量 (10 mg/kg)腹腔注射重复式染毒 ,每天 1次共 5天 ,于染毒后 2 4、4 8、72 h采集尾血进行网织红细胞微核分析。结果 外周血网织红细胞微核率呈现出良好的时间 -反应关系 ,微核的高峰出现在染毒后 4 8h,此时各剂量组微核率均高于对照组。低剂量重复染毒试验结果表明 ,在 2 4～ 72 h内网织红细胞微核率始终处于较高水平 ,与对照组相比差异有显著性 (P<0 .0 5 )。结论 铁路危险品货运站空气颗粒污染物对雄性小鼠外周血网织红细胞微核具有明显的诱导作用
Objective To investigate the induction effect of airborne particulate pollutants on the reticulocyte micronuclei in peripheral blood of mice in railway dangerous goods freight station. Methods Male BAL B / c mice were randomly divided into experimental group and control group. Acetone extracts of airborne particle contaminants were injected intraperitoneally at a dose of 20, 40, 80 and 160 mg / kg. poison. At the same time, low dose (10 mg / kg) intraperitoneal injection repeated exposure, once a day for 5 days, at 24,48,72 h after injection of tail blood collected for reticulocyte micronucleus analysis. Results The micronucleus rate of reticulocyte in peripheral blood showed a good time-response relationship. The peak of micronuclei appeared at 48 h after exposure. At this time, the micronucleus rate of each dose group was higher than that of the control group. The results of low dose repeated exposure test showed that the reticulocyte micronucleus rate was always at a high level within 24 h to 72 h, which was significantly different from the control group (P <0.05). Conclusions The airborne particulate pollutants at the railway dangerous goods terminal have significant inducing effects on the reticulocyte micronuclei in male mice