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报道了一种检测油脂中的合成抗氧剂(BHT、BHA、TBHQ和PG)MLC方法,含有抗氧剂的油脂按1∶4(Voil:V2-propanol)被稀释、过滤,并且在没有前期萃取的情况下直接注射进样分析。研究了抗氧剂的MLC方法的检测条件。当C18色谱柱、胶束流动相中含0.08MSDS,5%(V/V)异丙醇和0.01mol/LNaH2PO4.H2O,胶束流动相的pH为3～4,检测波长为280nm,流速1ml/min时,在1～15μg/g的范围内标准曲线呈线性关系(r>0.9992),4种抗氧剂的检测限为0.1～0.4μg/g。方法回收率为89.1%～99.9%。
A MLC method for the detection of synthetic antioxidants (BHT, BHA, TBHQ and PG) in fats and oils was reported. Fats containing antioxidants were diluted 1: 4 (Voil: V2-propanol), filtered, Direct extraction injection for injection analysis. The conditions for the MLC method of antioxidants were investigated. When C18 column, the micellar mobile phase contains 0.08MSDS, 5% (V / V) isopropanol and 0.01mol / L NaH2PO4.H2O, the pH of the micellar mobile phase is 3-4, the detection wavelength is 280nm, the flow rate is 1ml / min, the standard curve showed a linear relationship (r> 0.9992) in the range of 1 ~ 15μg / g, and the detection limit of four antioxidants was 0.1 ~ 0.4μg / g. The recovery rate was 89.1% ~ 99.9%.