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目的:动态观察氟哌啶醇季铵盐衍生物(F_3)对血管平滑肌细胞内钙浓度的影响。方法:利用激光共聚焦显微镜动态观察F_3(0.o1-10μmol/L)对由KCl(30mmol/L)诱导的大鼠血管平滑肌细胞钙荧光强度增加的作用。结果:KCl可诱发细胞内钙荧光强度迅速增强,F_3可以拮抗由KCl诱导的细胞内钙荧光强度增强作用,并呈量效依赖性和时间依赖性,终强度(KCl:67±24;F_3 0.o1μmol/L:57±13;0.1μmol/L:40±13;1μmol/L:29±9;10μmol/L:20±6)。在加入F_3后,钙荧光强度的变化最快时程是在给F_3后0-30s。结论:F_3拮抗血管收缩主要是由于阻断了Ca~(2+)通道。
Objective: To observe the effect of haloperidol quaternary ammonium salt derivative (F_3) on intracellular calcium concentration in vascular smooth muscle cells. Methods: Laser confocal microscopy was used to observe the effect of F_3 (0.1-10μmol / L) on the increase of calcium fluorescence in rat vascular smooth muscle cells induced by KCl (30mmol / L). RESULTS: KCl induced a rapid increase of intracellular calcium fluorescence intensity. F_3 could antagonize the increase of intracellular calcium fluorescence intensity induced by KCl, in a dose-dependent and time-dependent manner. The final intensity (KCl: 67 ± 24; F_3 0 .o1 μmol / L: 57 ± 13; 0.1 μmol / L: 40 ± 13; 1 μmol / L: 29 ± 9; 10 μmol / L: 20 ± 6). After adding F_3, the fastest change of calcium fluorescence intensity is 0-30s after giving F_3. Conclusion: F_3 antagonizes vasoconstriction mainly due to blocking of Ca 2+ channels.