论文部分内容阅读
目的 高效表达白细胞介素 2 (12 5Ser)。方法 采用新型Bac -to -Bac杆状病毒表达系统 ,以PCR技术扩增了人白细胞介素 2 (12 5Ser)的基因片段 ,将其克隆到转座载体 ,经转座作用将白细胞介素 2 (12 5Ser)基因插入杆状病毒穿梭载体 ,在昆虫细胞中表达。结果 通过免疫印迹实验及CTLL - 2细胞 /MTT法检测 ,表达产物具有人白细胞介素 2的免疫学及生物学活性。经SDS -PAGE电泳 ,薄层扫描分折 ,表达量达到了 2 3.47%。结论 白细胞介素 2 (12 5Ser)在昆虫细胞中获得了高效表达。
Objective To express interleukin 2 (125Ser) efficiently. Methods A novel Bac-to-Bac baculovirus expression system was used to amplify the human interleukin 2 (12 5Ser) gene fragment by PCR and cloned into a transposable vector. After transposition, interleukin 2 (125Ser) gene into the baculovirus shuttle vector for expression in insect cells. Results The results of immunoblotting assay and CTLL - 2 cell / MTT assay showed that the expressed product had the immunological and biological activities of human interleukin - 2. After SDS-PAGE electrophoresis, TLC scanning, expression reached 2 3.47%. Conclusion Interleukin-2 (125Ser) is highly expressed in insect cells.