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目的观察重组人转化生长因子-β1(rhTGF-β1)诱导人肺上皮细胞(A549)E-Cad蛋白表达的变化。方法将体外培养的A549用不同浓度的rhTGF-β1干预,48h后收集蛋白用Westerblot和间接免疫荧光方法检测上皮细胞表型E-钙黏蛋白(E-Cad)表达的变化。结果rhTGF-β1诱导A549下调上皮细胞表型E-Cad表达,并呈浓度依赖性。结论rhTGF-β1可诱导A549细胞E-Cad表达下调,支持在肺上皮细胞发生上皮细胞-间质转化(EMT)的观点。
Objective To observe the change of E-cad protein expression in human lung epithelial cells (A549) induced by recombinant human transforming growth factor-β1 (rhTGF-β1). Methods A549 cells cultured in vitro were treated with rhTGF-β1 at different concentrations. After 48 hours, the protein expression of E-cadherin in epithelial cells was detected by Westerblot and indirect immunofluorescence staining. Results rhTGF-β1 induced A549 down-regulated the phenotype E-Cad expression of epithelial cells in a concentration-dependent manner. Conclusion rhTGF-β1 can down-regulate the expression of E-cadherin in A549 cells and support the idea of epithelial-mesenchymal transition (EMT) in lung epithelial cells.