采用苦参合剂直接作用于隐孢子虫卵囊(CSO)后,用激光共焦显微技术(LCST)观察和处理。苦参合剂3份与羊抗兔荧光抗体液1份,充分混和成为药物标记液;用CSO液加等量的药物标记液(对照组加生理盐水)于37℃温育30min,离心取沉渣中CSO涂片,用金胺酚染色,将此样本运用MRC_(-600)LCST对CSO进行激光扫描,单元超薄切片的药物荧光密度会自动输入电脑进行二维面积或三维体积的量化比较处理。苦参合剂作用的CSO激发荧光 After treated with Sophora flavescens mixture directly on Cryptosporidium oocysts (CSO), they were observed and processed by laser confocal microscopy (LCST). 3 parts of Sophora flavescens mixture and 1 part of goat anti-rabbit fluorescent antibody solution were fully mixed into a drug labeling solution; CSO solution was added with the same amount of drug labeling solution (control group plus normal saline) and incubated at 37° C. for 30 minutes, and centrifuged to collect sediment. The CSO smear was stained with auramine, and the sample was scanned with MRC_(-600)LCST for laser scanning of the CSO. The fluorescence density of the ultrathin section of the unit was automatically entered into a computer for quantitative comparison of two-dimensional areas or three-dimensional volumes. CSO excited fluorescence of Sophora japonica mixture