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目的:建立了HPLC法测定复方磺胺甲噁唑片中磺胺甲噁唑与甲氧苄啶的含量。方法:采用ODS柱,以0.025mol·ml~(-1)磷酸溶液(用三乙胺调pH至3.0)—乙腈(80:20)为流动相,流速为1.Oml·min~(-1),检测波长为246nm。结果:磺胺甲噁唑与甲氧苄啶的线性范围分别为40.0～360.0μg·ml~(-1)和8.0～72.0μg·ml~(-1),平均回收率分别为99.8％(RSD=0.45％)和100.5％(RSD=0.26)。结论:本方法准确,快速,简便。
Objective: To establish a HPLC method for the determination of sulfamethoxazole and trimethoprim in compound sulfamethoxazole tablets. Methods: ODS column was used with a mobile phase of 0.025mol · ml -1 phosphoric acid (adjusted to pH 3.0 with triethylamine) -acetonitrile (80:20) at a flow rate of 1.0 ml · min -1 ), Detection wavelength of 246nm. Results: The linear ranges of sulfamethoxazole and trimethoprim were 40.0-360.0μg · ml -1 and 8.0-72.0μg · ml -1, respectively. The average recoveries were 99.8% (RSD = 0.45%) and 100.5% (RSD = 0.26). Conclusion: This method is accurate, fast and easy.