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采集广东肇庆市鼎湖山的野生型广州蛇根草,进行室内水培优化,并初步构建了广州蛇根草水培体系。采用HPLC和GC-MS技术分析鉴定根部分泌物中的喜树碱、金丝桃苷以及其他成分。结果表明:广州蛇根草水培体系需建立在无强阳光直射,温度适宜(18~25℃)的条件下。利用大孔树脂AB-8吸附结合固相萃取技术富集根系分泌液,以石油醚/乙酸乙酯(6∶4)为洗脱液进行柱色谱分离,获得浓缩液经HPLC检测含有喜树碱和金丝桃苷,表明广州蛇根草根系分泌物中存在喜树碱和金丝桃苷,同时GC-MS鉴定分析表明广州蛇根草根系分泌物中还含有各种热稳定的化感物质,如邻苯二甲酸二丁酯和苯二甲酸,单(2-乙基己基)酯等化合物。本研究初步表明通过构建蛇根草水培体系,以其根系生物合成喜树碱和金丝桃苷有一定的可行性。
The wild-type snakehead grass of Dinghushan, Zhaoqing City, Guangdong Province was collected for indoor hydroponics optimization, and the aquatic culture system of snakehead grass was initially constructed in Guangzhou. The camptothecin, hyperoside, and other constituents in the root exudates were identified by HPLC and GC-MS techniques. The results showed that the water solution system of P. sinica was established under the conditions of direct sunlight without strong temperature (18-25 ℃). The macroporous resin AB-8 adsorption coupled with solid-phase extraction was used to enrich the root exudates and the fractions were separated by column chromatography using petroleum ether / ethyl acetate (6: 4) as eluant. And hyperoside, indicating that there is camptothecin and hyperoside in the root exudates in Guangzhou snake grass, GC-MS identification analysis showed that the root exudates of Guangzhou snake grass also contained various thermostable allelochemicals , Such as dibutyl phthalate and phthalic acid, mono (2-ethylhexyl) esters and the like. The preliminary results of this study indicate that it is feasible to biologically synthesize camptothecin and hyperoside through its root system through the construction of the hydroponics system.