目的:探讨过表达的p27kip1对肝癌细胞(HHCC)的抑制作用。方法:利用脂质体介导法将真核表达载体pcDNA3.1/Myc-His(+)C-p27kip1基因导入HHCC中,用Westernblot检测p27kip1是否导入到HHCC中;用平板克隆形成实验、软琼脂克隆形成实验检测过表达的p27kip1对HHCC的抑制作用;应用流式细胞仪、电子显微镜探讨过表达的p27kip1抑制HHCC生长的可能机制。结果:Westernblot检测表明,转染p27kip1的HHCC100%表达p27kip1蛋白。过表达的p27kip1对HHCC有明显的抑制作用,抑制率为49.09%～56.00%;过表达的p27kip1可以封闭G1期的进程和诱导HHCC发生凋亡,凋亡百分比为12.3%。结论:过表达的p27kip1可以通过诱导凋亡抑制肝癌细胞生长。 Objective: To investigate the inhibitory effect of overexpressed p27kip1 on hepatoma cells (HHCC). METHODS: The eukaryotic expression vector pcDNA3.1/Myc-His(+)C-p27kip1 gene was introduced into HHCC by liposome-mediated method, and Western blot was used to detect whether p27kip1 was introduced into HHCC; plate cloning experiments and soft agar were performed. Clone formation assay was used to detect the inhibitory effect of overexpressed p27kip1 on HHCC; flow cytometry and electron microscopy were used to investigate the possible mechanism of overexpression of p27kip1 in inhibiting HHCC growth. RESULTS: Western blot analysis showed that 100% of HHCC transfected with p27kip1 expressed p27kip1 protein. Overexpression of p27kip1 significantly inhibited HHCC and the inhibition rate was 49.09%-56.00%. Overexpression of p27kip1 blocked the progression of G1 phase and induced HHCC apoptosis, with apoptotic percentage of 12.3%. CONCLUSIONS: Overexpression of p27kip1 can inhibit hepatoma cell growth by inducing apoptosis.