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通过平板培养的方式,观测不同培养温度、pH、碳源、氮源、碳氮源浓度组合对毛木耳Ⅰ菌丝生长的影响。结果表明,毛木耳Ⅰ菌丝生长的适宜温度为25~30℃,适宜pH为6~7,适宜碳源为果糖、蔗糖,适宜氮源为酵母膏、牛肉膏,适宜碳氮源浓度组合为果糖1%,蔗糖1%,酵母膏0.3%,牛肉膏0.3%。
The effects of different culture temperature, pH, carbon source, nitrogen source, concentration of carbon and nitrogen on mycelial growth of A. furcifera were observed by plate culture. The results showed that the suitable temperature for the growth of A. millet Ⅰ was 25-30 ℃ and the optimum pH was 6-7. The suitable carbon sources were fructose and sucrose. The suitable nitrogen source was yeast extract and beef extract, and the suitable combination of carbon and nitrogen sources was 1% fructose, 1% sucrose, 0.3% yeast extract and 0.3% beef extract.