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采用大鼠束缚－冷冻应激模型和小鼠无水乙醇损伤模型观察银杏叶提取物（ＧｂＥ）对胃粘膜损伤指数的影响；采用幽门结扎法收集胃液，观察ＧｂＥ对胃液分泌量，胃液酸度和胃蛋白酶活性的影响；采用硫代巴比妥酸（ＴＢＡ）法测定胃粘膜及血清中丙二醛（ＭＤＡ）含量。结果表明，ＧｂＥ（２５，５０，１００ｍｇ／ｋｇ，ｂｉｄ×５ｄ，ｉｇ）可剂量依赖性地抑制束缚－冷冻应激（ＲＣＳ）和无水乙醇引起的胃粘膜损伤。用药组应激后的胃粘膜损伤指数分别为对照组的５８．１１％，４２．９９％和３１．２３％；用药组乙醇诱发的胃粘膜损伤指数降至对照组的６１．５４％，３６．０６％和２５．６４％；ＧｂＥ尚能增强西米替丁对胃粘膜的保护作用。但对大鼠胃液分泌量、胃液酸度及胃蛋白酶活性ＧｂＥ并无明显影响。小鼠经无水乙醇ｉｇ后１ｈ，胃粘膜和血清中的ＭＤＡ含量显著升高（Ｐ＜０．０１），而ＧｂＥ（２５，５０，１００ｍｇ／ｋｇｉｇ）预处理则可以明显抑制ＭＤＡ的升高。因此认为ＧｂＥ具有胃粘膜保护作用，并且与西米替丁在治疗胃溃疡方面具有协同作用，这些作用可能与其抗氧化作用相关。
The effects of Ginkgo biloba extract (GbE) on the gastric mucosal injury index were studied using a rat restraint-freezing stress model and a mouse model of absolute ethanol injury. Gastric fluid was collected by pylorus ligation to observe the secretion of GbE to gastric juice, and the acidity of gastric juice was measured. Effects of pepsin activity; thiobarbituric acid (TBA) method was used to measure malondialdehyde (MDA) content in gastric mucosa and serum. The results showed that GbE (25, 50, 100 mg/kg, bid x 5 d, ig) dose-dependently inhibited gastric mucosal injury induced by binding-freezing stress (RCS) and absolute ethanol. The index of gastric mucosal injury after treatment was 58.11%, 42.99% and 31.23% of that in the control group, and the index of alcohol-induced gastric mucosal injury in the drug group was reduced to 61.54% in the control group. 36 .06% and 25.64%; GbE can still enhance the protective effect of cimetidine on gastric mucosa. However, there was no significant effect on gastric juice secretion, gastric acidity, and pepsin activity in rats. The content of MDA in gastric mucosa and serum was significantly increased (P<0.01) after 1h of ethanol administration in mice, but pretreatment with GbE (25, 50, 100 mg/kgig) could significantly inhibit the increase of MDA. . Therefore, it is considered that GbE has gastric mucosal protection and synergy with cimetidine in the treatment of gastric ulcer, and these effects may be related to its antioxidative effect.