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该实验以落新妇苷为内参物,研究一测多评法同时测定黔产侗药“钻更”药材中落新妇苷、黄杞苷的含量。在HPLC和UPLC的2个平台上,通过不同型号仪器相对校正因子的考察,探讨不同色谱体系之间所建立的相对校正因子的互用性,再根据建立的相对校正因子计算黄杞苷的含量,并与外标一点法、外标工作曲线法进行比较,以验证一测多评法的准确性。结果表明,在不同色谱体系中,落新妇苷与黄杞苷的相对校正因子及保留时间比值的重复性良好,RSD分别为2.0%,1.8%,均小于3.0%,侗药“钻更”药材中黄杞苷的一测多评法计算值与外标一点法、外标工作曲线法的相对偏差为1.6%~3.9%,RSD2.0%~0.80%,符合相关要求,Pearson相关系数为0.999 8(P<0.01)。研究表明,采用一测多评法分析黔产侗药“钻更”药材中落新妇苷、黄杞苷的含量信息,该方法准确、可靠、重复性良好,可较好地控制该药材的内在质量。
In this experiment, astilbin was used as an internal reference to study the determination of asparticin and huangqi glycosides in Qianshi Dong medicine On the two platforms of HPLC and UPLC, the relative correction factors of different types of instruments were investigated to explore the interoperability of the relative correction factors established between different chromatographic systems, then the content of flavonoids was calculated based on the established relative correction factors , And compared with the external standard one point method and external standard working curve method to verify the accuracy of the multiple test method. The results showed that in different chromatographic systems, the relative correction factor and retention time ratio of astilbin and huang glycoside were good, with RSDs of 2.0% and 1.8%, respectively, less than 3.0% The relative deviation between the calculated value of the external reference standard and the standard method of the external standard of 1.6 to 3.9% and RSD 2.0% to 0.80% of the measured values of the flavonoid glycosides in the medicinal materials meet the relevant requirements. The Pearson correlation coefficient Was 0.999 8 (P <0.01). The results showed that the content of astilbin and huangqi glycosides in Qian medicine “DRG” was analyzed by a multi-evaluation method. The method is accurate, reliable and reproducible, and can be used to control the medicine The intrinsic quality.