论文部分内容阅读
从广东省南澳县西山等养蛙场多次收集患黄杆菌病牛蛙,其症状是:歪头、狂游、双眼白内障和腹水等。从病蛙脑浆、眼底和腹水中分离到多株病原菌,急性毒性试验表明病原菌对健康牛蛙致病力强。取有代表性的981007-2等菌株进行系统的生理生化试验显示,革兰氏染色阴性、无鞭毛、无芽孢的短杆菌;分离菌株内毒素检验阳性;病原菌显示了对青霉素类药物高度耐药、好氧型、利用葡萄糖等生理、生化特性。经VITEK-AMS-60自动化检测系统鉴定为黄杆菌IIb群(Plavobacterium group IIb)。选用47种抗菌药物,通过K-B纸片扩散法对病原菌进行耐药性研究结果,分离菌株对38种抗菌药物显示耐药(占80.85％)、对4种药物中等敏感(占8.51％),仅对利福平、环丙沙星、氧氟沙星、氟啶酸、麦迪霉素等5种药物(占10.64％)敏感。应用试管稀释法测定上述5种敏感药物的平均MIC分别是0.19、0.27、0.53、2.40和10.2μg/ml。
Nanfang County, Guangdong Province, Xishan and other raised frog field repeatedly collected flagellates bullfrog disease, its symptoms are: tilt head, wandering, cataracts and ascites and so on. From sick frog brain pulp, fundus and ascites were isolated from many strains of pathogens, acute toxicity tests showed that pathogenic bacteria on the strong bullfrog virulence. Take the representative of the 981007-2 and other strains of the system of physiological and biochemical tests showed that Gram-negative, flagella, non-sporeless Brevibacterium; isolated strains endotoxin test positive; pathogenic bacteria showed high resistance to penicillins , Aerobic, the use of glucose and other physiological and biochemical characteristics. It was identified as Plavobacterium group IIb by VITEK-AMS-60 automated detection system. Forty-seven kinds of antibacterials were selected and tested for their resistance to pathogenic bacteria by KB disk diffusion method. The isolates showed resistance to 38 antibiotics (80.85%), moderate sensitivity to 4 drugs (8.51%), and only Right rifampicin, ciprofloxacin, ofloxacin, uridine acid, midecamycin and other five drugs (10.64%) sensitive. The average MICs of the five sensitive drugs measured by in vitro dilution were 0.19, 0.27, 0.53, 2.40 and 10.2 μg / ml, respectively.