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近年的研究表明,肿瘤坏死因子（TNF）、白细胞介素-2（IL-2）与肿瘤的发生、发展及预后有密切的关系。为了了解食管癌患者TNF及IL-2含量的变化。我们检测了食管癌患者血清中TNF、IL-2含量,并就临床意义作初步探讨。 对象和方法 一、对象: （一）正常人:35人。均为我院门诊体检合格的健康人。肝、肾功能试验正常。 （二）病人组:31例。均选自本院住院患者,全部病例均经临床明确诊断（包括X线、部分病例经病理切片证实）。患者在手术前和手术后2周分别抽取静脉血,当日分离血清-20℃保存待测,一周内测定完毕。 二、方法: （一）TNF:RIA法。试剂盒由解放军总医院东亚免疫试剂研究所提供,按说明书进行。 （二）IL-2:RIA法。试剂盒由上海长征医学科学有限公司提供,按说明书进行。 结果 正常人和食管癌患者手术前TNF、IL-2含量测定结果见表1。 二、食管癌患者手术后二周与正常人血清中TNF、IL-2含量测定结果见表2。
Recent studies have shown that tumor necrosis factor (TNF), interleukin-2 (IL-2) are closely related to the occurrence, development and prognosis of tumors. To understand the changes of TNF and IL-2 levels in esophageal cancer patients. We measured the serum levels of TNF and IL-2 in patients with esophageal cancer and made a preliminary study on clinical significance. Objects and Methods 1. Objects: (1) Normal people: 35 people. All of them were healthy people who passed medical examination in our hospital. Liver and kidney function tests were normal. (b) Patient group: 31 cases. All patients were selected from hospitalized patients in this hospital, all cases were confirmed by clinical diagnosis (including X-ray, some cases were confirmed by pathological section). Patients received venous blood before surgery and 2 weeks after surgery. The serum was separated and stored at -20°C on the same day and was determined within one week. Second, methods: (A) TNF: RIA method. The kit was provided by the East Asia Immunological Reagent Institute of the General Hospital of the People’s Liberation Army and was performed according to the instructions. (b) IL-2: RIA Act. The kit was provided by Shanghai Changzheng Medical Science Co., Ltd. and the instructions were followed. Results Table 1 shows the results of TNF and IL-2 determination before surgery in normal people and esophageal cancer patients. Second, the results of the determination of TNF and IL-2 levels in serum of normal and esophageal patients two weeks after surgery are shown in Table 2.