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目的:建立重建膀胱感觉和运动神经支配的SD大鼠模型并进行鉴定,为进一步研究排尿中枢重塑及其机制奠定基础。方法:45只雌性SD大鼠随机分为对照组(n=10)、神经根切断组(n=15)和神经根吻合组(n=20)。神经根切断组大鼠,将L4以下双侧脊神经前后根全部切断;神经根吻合组在切断脊神经根后,将双侧L4神经前后根与S1相应神经根吻合;对照组不作手术处理。术后6个月,取各组大鼠,分别行尿流动力学检测、神经根电刺激、神经吻合口甲苯胺蓝染色、盆神经节注射荧光金神经示踪染色和膀胱湿质量测量。结果:神经根吻合组大鼠膀胱最大容量、残余尿量、膀胱顺应性及膀胱湿质量均小于神经根切断组而大于对照组(P<0.05);神经根吻合组大鼠最大排尿压与对照组比较差异无统计学意义(P>0.05),但大于神经根切断组(P<0.05)。神经根吻合组电刺激神经根后膀胱内压升高,但低于对照组(P<0.05)。神经根吻合组神经吻合口甲苯胺蓝染色可见神经纤维通过率达(53.4±6.7)%。盆神经节内注射荧光金后,神经根吻合组可见L4脊髓节段双侧灰质荧光金染色,神经根切断组和对照组未在相应脊髓节段检测到荧光金染色。结论:成功建立了同时重建膀胱感觉及运动神经支配的大鼠动物模型,为进一步研究排尿中枢重塑及其机制奠定了基础。
OBJECTIVE: To establish and identify a model of SD rats with bladder sensation and motor innervation and to establish a basis for further study on urinary central remodeling and its mechanism. Methods: Forty-five female SD rats were randomly divided into control group (n = 10), nerve root excision group (n = 15) and nerve root anastomosis group (n = 20). Rats in the nerve root transection group were severed all the anterior and posterior roots of the bilateral spinal nerves below L4. After the root of the radiculotomy was cut off, the anterior and posterior roots of the bilateral L4 nerves were anastomosed with the corresponding nerve roots of S1. The control group was not treated surgically. Six months after operation, the rats in each group were subjected to urodynamic tests, electrical nerve root stimulation, toluidine blue staining of the anastomosis of the nerves, fluorescent gold nerve tracer staining of the pelvic ganglion and measurement of bladder wet mass. Results: The maximal urinary bladder volume, residual urine volume, bladder compliance and bladder wet mass in the nerve root anastomosis group were significantly lower than those in the control group (P <0.05). The maximal urinary pressure in the nerve root anastomosis group was significantly higher than that in the control group There was no significant difference between the two groups (P> 0.05), but higher than that of the nerve root excision group (P <0.05). In the nerve root anastomosis group, the intravesical pressure increased after nerve root stimulation, but lower than that in the control group (P <0.05). Nerve root anastomotic nerve root anastomosis toluidine blue staining showed nerve fiber through rate (53.4 ± 6.7)%. Fluorogold was injected into the pelvis ganglion, and bilateral gray matter fluorescence staining of the L4 spinal cord segment was observed in the root-mean-square nerve root ganglion. Fluorescent gold staining was not detected in the corresponding spinal cord segments of the nerve root excision group and the control group. Conclusion: The establishment of a successful animal model of rebuild bladder sensory and motor innervation at the same time, which laid the foundation for the further study of urinary central remodeling and its mechanism.