目的:探讨Foxm1在非小细胞肺癌细胞EMT过程中的作用进而研究其对非小细胞肺癌细胞迁移和浸润能力的影响。方法:采用Western blot和RT-PCR技术检测不同非小细胞肺癌细胞系中Foxm1和EMT相关因子的表达水平。小RNA转染技术干扰H1299细胞中Foxm1的表达。Transwell试验观察Foxm1对非小细胞肺癌细胞迁移浸润能力的影响。结果:Foxm1在四种非小细胞肺癌细胞中均有表达,且H1299表达量相对较高,H1650表达量相对较低。分别以上述两种细胞为试验对象,结果显示Foxm1的表达与EMT相关分子E-cadherin、Vimentin的表达呈明显相关性。CCK-8结果显示:Foxm1抑制剂Thiostrepton处理72h后对H1299、H1650的IC50值分别为1.21μmol/L和3.08μmol/L。Thiostrepton处理和干扰后,Foxm1和Vimentin的表达量明显下降。Transwell小室迁移试验24h后,空白组、阴性对照组和干扰组穿膜细胞数分别为42.6±6.9,36.3±4.2,4.6±1.1;侵袭试验24h后,空白组、阴性对照组和干扰组穿膜细胞数分别为12.3±3.4,10.0±1.4,3.1±2.6。结论:Foxm1在非小细胞肺癌细胞中可能通过调节EMT进程来促进肿瘤细胞的迁移和浸润能力。 OBJECTIVE: To investigate the role of Foxm1 in the process of EMT in non-small cell lung cancer cells and to investigate its effect on the migration and invasion of non-small cell lung cancer cells. Methods: Western blot and RT-PCR were used to detect the expression of Foxm1 and EMT in different non-small cell lung cancer cell lines. Small RNA transfection technology interferes with Foxm1 expression in H1299 cells. Transwell assay was used to observe the effect of Foxm1 on migration and invasion of non-small cell lung cancer cells. Results: Foxm1 was expressed in all four non-small cell lung cancer cell lines, with relatively high expression of H1299 and relatively low expression of H1650. The above two kinds of cells were used as experimental subjects respectively. The results showed that the expression of Foxm1 was significantly correlated with the expression of E-cadherin and Vimentin. The results of CCK-8 showed that IC50 values ​​of H1299 and H1650 were 1.21μmol / L and 3.08μmol / L respectively after 72h treatment with Foxm1 inhibitor Thiostrepton. After Thiostrepton treatment and interference, Foxm1 and Vimentin expression decreased significantly. The number of transmembrane cells in the blank group, the negative control group and the interference group was 42.6 ± 6.9, 36.3 ± 4.2 and 4.6 ± 1.1 respectively after transwell chamber migration test for 24 h. After the invasion assay for 24 h, the blank group, the negative control group and the interference group penetrated the membrane Cell numbers were 12.3 ± 3.4, 10.0 ± 1.4, 3.1 ± 2.6, respectively. Conclusion: Foxm1 may promote the migration and invasion of tumor cells in non-small cell lung cancer cells by regulating EMT progression.