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目的:探讨三皮汤对溃疡性结肠炎(UC)小鼠的疗效及可能治疗机制。方法:40只BALB/C小鼠随机分成正常组、模型组、柳氮磺胺吡啶(SASP)组和三皮汤组,每组10只。予以恶唑酮建立溃疡性结肠炎模型后,三皮汤组和SASP组分别给予三皮汤和SASP灌胃治疗,每天观察一般情况,十天后处死,剪取脾组织检测重量的变化,取其病变结肠观察组织学改变,并采用ELISA测定各组小鼠血清和脾脏组织细胞因子IFN-γ及IL-4的含量。结果:三皮汤组和SASP组小鼠症状较模型组有明显改善;模型组小鼠免疫器官脾组织明显萎缩,三皮汤组和SASP组小鼠脾组织重量较模型组有所增加;三皮汤组血清和脾脏组织IFN-γ含量低于模型组(P<0.01),IL-4含量高于模型组(P<0.01)。结论:三皮汤能通过降低IFN-γ及升高IL-4的含量,来调整细胞因子间的网络平衡,改善机体免疫功能,缓解肠道炎症反应。
Objective: To investigate the effect of Sanpi Decoction on ulcerative colitis (UC) mice and the possible therapeutic mechanism. Methods: Forty BALB / C mice were randomly divided into normal group, model group, sulfasalazine (SASP) group and Sanpi decoction group, with 10 mice in each group. After establishing the model of ulcerative colitis with oxazolone, Sanpi Decoction group and SASP group were treated with Sanpi Decoction and SASP respectively. The general situation was observed daily. Ten days later, the rats were sacrificed and the changes of the weight of the spleen tissue were measured. The pathological changes of colon were observed. The contents of cytokines IFN-γ and IL-4 in serum and spleen of mice in each group were determined by ELISA. Results: Compared with the model group, the symptoms of mice in the three-Pi Tang group and the SASP group were significantly improved. The spleen of the mice in the model group was significantly reduced. The weight of spleen in the Sanpi Tang and SASP groups was increased compared with the model group. The content of IFN-γ in serum and spleen of Pi Tang group was lower than that of model group (P <0.01), and the content of IL-4 was higher than that of model group (P <0.01). Conclusion: Sanpi Decoction can regulate the network balance of cytokines by decreasing the content of IFN-γ and increasing the content of IL-4, improve the body’s immune function and relieve the intestinal inflammatory reaction.